Torab Ghanbari; Rahim Abdi; Mahmood Bahmani; Rezvanollah Kazemi; Ahmad Savari
Volume 5, Issue 2 , December 2016, , Pages 17-23
Abstract
In this study cartilage tissue of vertebral column and dorsal fin of 40 days, 70 days, one and 4 years old of Persian sturgeon after preparation were fixed in buen fixative. Then rutin procedure of histologicql examination done and paraffin blocks cut at 6 microne and then stained with H&E, Safranin-O ...
Read More
In this study cartilage tissue of vertebral column and dorsal fin of 40 days, 70 days, one and 4 years old of Persian sturgeon after preparation were fixed in buen fixative. Then rutin procedure of histologicql examination done and paraffin blocks cut at 6 microne and then stained with H&E, Safranin-O and Verhoff staining. In photomicrographs were compared cell structure and compounds of substance grounds in cartilage tissues. It showed that perichondrium layer of dorsal fin was more advanced from perichondrium layer of vertebral column and their mixture were different. Microscopic masurement of two cartilage tissue showed the cells of dorsal fin were larger than the cells of vertebral column but in cells number, decrised with physiological maturation. Comparative study of volume of extracellular matrix and number of isogenous groups by SPSS software showed that growth of cartilage tissue of vertebral column was interstitial but in dorsal fin was appositional.
T. Ghanbari; R. Abdi; M. Bahmani; R. Kazemi; A. Savari
Volume 3, Issue 4 , July 2015, , Pages 1-8
Abstract
Abstract In this study, cartilage tissue of vertebral column and dorsal fin of 40 days, 70 days, one and 4 years old of Persian sturgeon after preparation were fixed in buen fixative. Then rutin procedure of histology examination were done and paraffin blocks cut at 6 microne and then stained ...
Read More
Abstract In this study, cartilage tissue of vertebral column and dorsal fin of 40 days, 70 days, one and 4 years old of Persian sturgeon after preparation were fixed in buen fixative. Then rutin procedure of histology examination were done and paraffin blocks cut at 6 microne and then stained with H&E, Safranin-O and Verhoff staining. In photomicrographs were compared cell structure and compounds of substance grounds in cartilage tissues. It showed that perichondrium layer of dorsal fin was more advanced from perichondrium layer of vertebral column and their componants were different. Microscopic masurement of two cartilage tissue showed the cells of dorsal fin were larger than the cells of vertebral column but in cells number,decrised with physiological maturation. Comparative study of volume of extracellular matrix and number of isogenous groups by SPSS software showed that growth of cartilage tissue of vertebral column was interstitial but in dorsal fin was appositional.
A Mir-Aali; A A. Movahedi-Nia; R Abdi; Salati M. P.
Volume 3, Issue 1 , January 2015, , Pages 59-64
Abstract
Abstract 144 juvenile Sobaity, Sparidentex hasta, after acclimation to laboratory conditions and sea water (40ppt) for one week, sea water was used with different salinities (5, 20 and 60ppt) and seawater (40ppt) as control. Samplings were performed at 6 and 12 hours, 1, 2, 7 and 14 days after the time ...
Read More
Abstract 144 juvenile Sobaity, Sparidentex hasta, after acclimation to laboratory conditions and sea water (40ppt) for one week, sea water was used with different salinities (5, 20 and 60ppt) and seawater (40ppt) as control. Samplings were performed at 6 and 12 hours, 1, 2, 7 and 14 days after the time of changing in environmental salinities. Blood samples were collected for plasma cortisol, glucose and electrolytes analysis. According to the results, plasma levels of cortisol showed significant increases in 5 and 60 ppt environment in relation to control and 20ppt groups 12 hours from the beginning of the experiment. However in 24h samples up to the end of the experiment (14days) there were no significant differences among treatments. Plasma glucose levels showed a significant increase only at 12h sampling time in relation to 6 and 24hours in fish adapted to 5 and 60ppt. Plasma Ca++ concentrations had no significant changes during experiment. Plasma Mg++ amounts decreased significantly in 5 and 20 ppt groups in relation with control at 24 h sampling time. In conclusion, cortisol has important role in adaptation to both 5 and 60ppt at the beginning of the exposure, because of its significant increase at just 12 hours in 5 and 60 ppt than the controls, but the role of cortisol has been replaced with other changes in osmoregulatory related tissues and cells according to the plasma cortisol amounts in 5 and 60ppt that returned to the basic levels.
